Technical Data
A0859-44P
APPL2 (DCC-interacting Protein 13-beta, Dip13-beta, Adapter Protein Containing PH Domain, PTB Domain and Leucine Zipper Motif 2, DIP13B)
Description:
APPL2, also termed as Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2, has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, APPL1, adiponectin receptors, and proteins of the NuRD/MeCP1 complex.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1-2ug/ml
Western Blot: 0.2-0.3ug/ml
Immunohistochemistry: 2-3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Recombinant full-length human APPL2 expressed in E.coli.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA.
Specificity:
Recognizes human APPL2. Species Cross-Reactivity: monkey, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.