Technical Data
A0859-55P
Adducin, alpha (Alpha-adducin, Erythrocyte Adducin Subunit alpha, ADD1, ADDA)
Description:
Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes. Adducin is a heterodimeric protein that consists of related subunits; alpha and beta, or alpha and gamma subunits. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha.

Applications:
Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence:1:100-1:200
Western Blot:1:500-1:1,000
Immunohistochemistry:1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic non-phosphopeptide corresponding to human ADD1 around the phosphorylation site of serine 726 (T-P-S P-F-L).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human ADD1. Species Cross-Reactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.