Technical Data
A0859-55S
Adducin, alpha (Alpha-adducin, Erythrocyte Adducin Subunit alpha, ADD1, ADDA)
Description:
ADD1 is a ubiquitously expressed membrane-skeletal protein localized at spectrin-actin junctions that binds calmodulin and is an in vivo substrate for protein kinase C (PKC) and Rho-associated kinase. ADD1 is a tetramer comprised of either f/g or f/n heterodimers. ADD1 subunits are related in sequence and all contain an N-terminal globular head domain, a neck domain and a C-terminal protease-sensitive tail domain. The tail domains of all ADD1 subunits end with a highly conserved 22-residue myristoylated alanine-rich C kinase substrate (MARCKS)-related domain that has homology to MARCKS protein. It caps the fast-growing ends of actin filaments and also preferentially recruits spectrin to the ends of filaments. Recent observations suggest a role for the protein in cell motility, and as a target for regulation by Rho-dependent and Ca2+-dependent pathways. Human ADD1 gene is mapped to chromosomal region 4p16.3.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceMouseRabbit
Concentration:
Not determined
Immunogen:
Recombinant corresponding to mouse ADD1.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity:
Recognizes mouse ADD1. Species sequence homology: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.