Technical Data
A0859-60D
ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motif 4) (Biotin)
Description:
ADAMTS4, also known as aggrecanase-1, is a secreted member of the ADAM family of proteins. ADAMTS4 is upregulated by TNF-alpha in osteoarthritis and contributes to joint destruction. Its upregulation in some cancers may enhance tumor invasiveness. Human ADAMTS4 consists of a signal peptide (aa1-51), a pro domain (aa52-212) and a mature chain (aa213-837) containing the following domains: catalytic (aa218-425), disintegrin (aa437-519), TSP type-1 (aa520-575), Cys-rich (aa 577 to 685) and spacer (aa686-837). The amino acid sequence of rhADAMTS4 is 100%, 97% and 94% identical to that of chimpanzee, dog, and mouse/rat/bovine.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 1-2ug/ml. The detection limit for rhADAMTS4 is approximately 5ng/lane under non-reducing and reducing conditions.
Immunoprecipitation: 25ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgG2aAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanGoat
Concentration:
~50ug/ml
Immunogen:
NS0-derived, recombinant human ADAMTS4 aa213-685.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 50ug BSA, 5% trehalose. Reconstitute with sterile Tris-buffered saline pH 7.3 (20mM Tris base, 150mM sodium chloride) containing 0.1% BSA. If 1ml of buffer is used, the antibody c
Specificity:
Recognizes human ADAMTS4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.