Technical Data
A0859-90B
Adaptin, alpha (AP-2 Complex Subunit alpha-1, 100kD Coated Vesicle Protein A, Adapter-related Protein Complex 2 alpha-1 Subunit, Adaptor Protein Complex AP-2 Subunit alpha-1, Alpha-adaptin A, Alpha1-adaptin, Clathrin Assembly Protein Complex 2 alpha-A Lar
Description:
Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC) and alpha-adaptin A and C. Coat proteins of approximately 100kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with 1.2% sodium acetate. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a10K99Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceBovineMouse
Concentration:
As reported
Immunogen:
AP-2 adaptor polypeptides from bovine brain.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
Specificity:
Recognizes Adaptin, alpha. Species Crossreactivity: human, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Robinson, M. S. : Cloning of cDNAs encoding two related 100kD coated vesicle proteins (alpha-adaptins). J. Cell Biol. 108: 833-42, 1989, 2. Waelter, S.; Scherzinger, E.; Hasenbank, R.; Nordhoff, E.; Lurz, R.; Goehler, H.; Gauss, C.;Sathasivam, K.; Bates, G. P.; Lehrach, H.; Wanker, E. E. : The huntingtin interacting protein HIP1 is a clathrin and alpha-adaptin-binding protein involved in receptor-mediated endocytosis. Hum. Molec. Genet. 10: 1807-1817, 2001.