		Technical Data
A0859-90B	Adaptin, alpha (AP-2 Complex Subunit alpha-1, 100kD Coated Vesicle Protein A, Adapter-related Protein Complex 2 alpha-1 Subunit, Adaptor Protein Complex AP-2 Subunit alpha-1, Alpha-adaptin A, Alpha1-adaptin, Clathrin Assembly Protein Complex 2 alpha-A Lar

Description:
Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC) and alpha-adaptin A and C. Coat proteins of approximately 100kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 1.2% sodium acetate. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	10K99	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Bovine	Mouse

Concentration:

As reported

Immunogen:

AP-2 adaptor polypeptides from bovine brain.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.

Specificity:

Recognizes Adaptin, alpha. Species Crossreactivity: human, rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Robinson, M. S. : Cloning of cDNAs encoding two related 100kD coated vesicle proteins (alpha-adaptins). J. Cell Biol. 108: 833-42, 1989, 2. Waelter, S.; Scherzinger, E.; Hasenbank, R.; Nordhoff, E.; Lurz, R.; Goehler, H.; Gauss, C.;Sathasivam, K.; Bates, G. P.; Lehrach, H.; Wanker, E. E. : The huntingtin interacting protein HIP1 is a clathrin and alpha-adaptin-binding protein involved in receptor-mediated endocytosis. Hum. Molec. Genet. 10: 1807-1817, 2001.