Technical Data
Adeno-Associated Virus 2 (AAV2)
Suitable for use in Immunofluorescence microscopy, Immunohistochemistry, Immunoprecipitation, Neutralization Assay, Affinity chromatography and ELISA. Not suitable for use in Immunoblot. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:20
Immunoprecipitation: 1:5
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C. Stable for 12 months after receipt at 4C. Reconstitute with sterile ddH2O. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG35F148Affinity Purified
50ug4C Do not freezeBlue IceMouse
~0.05mg/ml (after reconstitution)
Adeno-associated virus capsid proteins and virus particles.
Purified by Protein A affinity chromatography.
Supplied as a lyophilized powder from PBS, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Recognizes AAV-2. Species Crossreactivity: human and monkey. Reacts with AAV-3. Specifically reacts wtih intact AAV particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids not present in denatured capsid proteins and native but unassembled capsid proteins. Major reaction attributed to aa369-378 of AAV-2 capsids.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Wistuba A.,Weger S., Kern A., and Kleinschmidt J. (1995) Intermediates of adeno-associated virus type 2 assembly: Identification of soluble complexes containing Rep and Cap proteins. J. Virol. 69, 5311-5319.
Wistuba A., Kern A., Weger S., Grimm D., and Kleinschmidt J. (1997) Subcellular compartmentalization of adeno-associated virus type 2 assembly. J. Virol. 71, 1341-1352. Grimm D, Kern A, Pawlita M, Ferrari F, Samulski R, Kleinschmidt J (1999) Titration of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant AAV-2. Gene Ther 6(7) 1322-1330. Wobus C.A., Hgle-Drr B., Girod A., Petersen G., Hallek M. and Kleinschmidt J.A. (2000) Monoclonal antibodies against the adenoassociated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virology 74, 9281-9293.