Technical Data
A0868-36K
Adeno-Associated Virus (AAV) VP1, VP2, VP3
Description:
Useful for immunolocalization studies of capsid formation in combination with monoclonal A0868-35 and rabbit polyclonal antibody to AAV capsid proteins (Cat. % A0868-36N). Clone B1 reacts with free VP 1, VP 2 and VP 3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wo- bus et al., see below) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation, and Affinity chromatography. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1:10 Fix with formaldehyde. Incubation Time 1 hr at RT.
Immunoprecipitation: Mainly free VP proteins.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS or TBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1B1Affinity Purified
SizeStorageShippingSourceHost
50ug 4C (-20C Glycerol)Blue IceMouse
Concentration:
~0.05mg/ml (after reconstitution)
Immunogen:
Adeno-associated virus capsid proteins and virus particles.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder in 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile PBS or TBS.
Specificity:
Recognizeds AAV-2 found in human and monkey. Cross-reactive with AAV-1, AAV-3, AAV-4, AAV-5 and AAV-6 (weak cross-reactivity with AAV-4, AAV-5, AAV-11 and AAV-12).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Wistuba A.,Weger S., Kern A., and Kleinschmidt J. (1995) Intermediates of adeno-associated virus type 2 assembly: Identification of soluble complexes containing Rep and Cap proteins. J. Virol. 69:, 5311-5319. 2. Wistuba A., Kern A., Weger S., Grimm D., and Kleinschmidt J. (1997) Subcellular compartmentalization of adeno-associated virus type 2 assembly. J. Virol. 71:1341-1352. 3. Wobus C.A., Hgle-Drr B., Girod A., Petersen G., Hallek M. and Kleinschmidt J.A. (2000) Monoclonal antibodies against the adenoassociated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virology 74: 9281-9293.