Description:
Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation, and Affinity chromatography. Other applications have not been tested.
Recommended Dilution:
Immunohistochemistry: 1:10 Fix with formaldehyde. Incubation Time 1 hr at RT.
Immunoprecipitation: Mainly free VP proteins.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding 40-50% glycerol, ddH20. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 5F165 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 50ug | | 4°C (-20°C Glycerol) | Blue Ice | | Mouse |
|
| Concentration: |
| ~0.05mg/ml |
| Immunogen: |
| Adeno-associated virus capsid proteins and virus particles. Epitope: 726 to aa733 (C-terminus; common to all 3 VP proteins). |
| Purity: |
| Purified by Protein A affinity chromatography. |
| Form |
| Supplied as a lyophilized powder in 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml 40-50% glycerol, ddH20. |
| Specificity: |
| Recognizeds AAV-2 found in human and monkey, and all published AAV serotypes (AAV-4 only weak cross-reaction). Combination with monoclonal antibody and rabbit polyclonal antibody to AAV capsid proteins. Reacts with free VP 1, VP 2 and VP 3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1.Wistuba A.,Weger S., Kern A., and Kleinschmidt J. (1995) Intermediates of adeno-associated virus type 2 assembly: Identification of soluble complexes containing Rep and Cap proteins. J. Virol. 69:, 5311-5319. 2. Wistuba A., Kern A., Weger S., Grimm D., and Kleinschmidt J. (1997) Subcellular compartmentalization of adeno-associated virus type 2 assembly. J. Virol. 71:1341-1352. 3. Wobus C.A., Hügle-Dörr B., Girod A., Petersen G., Hallek M. and Kleinschmidt J.A. (2000) Monoclonal antibodies against the adenoassociated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virology 74: 9281-9293.
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