		Technical Data
A0868-37A	Adeno-Associated Virus 2 Replicase (AAV2 Replicase)

Description:
Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:50-1:100; with ECL dilute further.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	5F92	Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice		Mouse

Concentration:

~0.05mg/ml

Immunogen:

AAV Replicase

Purity:

Purified IgG

Form

Supplied as a lyophilized powder. Reconstitute to 1ml final volume.

Specificity:

Reactive with AAV-2, found in human and monkey. Recognizes AAV replicase proteins Rep 78, Rep 68, Rep 52 and Rep 40 in human AAV-2-infected cells. Crossreactivity with AAV serotypes 1, 3, 4, 5, and 6. A weak crossreactivity is seen in some human cell lines.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Wistuba A, Weger S, Kern A, and Kleinschmidt J. (1995). Intermediates of adeno-associated virus type 2 assembly: Identification of soluble complexes containing Rep and Cap proteins. J Virol 69, 5311-5319