Suitable for use in Western Blot. Other applications not tested.
Western Blot: 1:50-1:100; with ECL dilute further.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Supplied as a lyophilized powder. Reconstitute to 1ml final volume.|
|Reactive with AAV-2, found in human and monkey. Recognizes AAV replicase proteins Rep 78, Rep 68, Rep 52 and Rep 40 in human AAV-2-infected cells. Crossreactivity with AAV serotypes 1, 3, 4, 5, and 6. A weak crossreactivity is seen in some human cell lines.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Wistuba A, Weger S, Kern A, and Kleinschmidt J. (1995). Intermediates of adeno-associated virus type 2 assembly: Identification of soluble complexes containing Rep and Cap proteins. J Virol 69, 5311-5319 |