Technical Data
A0868-37B
Adeno-Associated Virus 2 Replicase (AAV2 Replicase)
Description:
Applications:
Suitable for use in Immunoblot.

Recommended Dilution:
Immunoblot: 1:50-1:100

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15F106Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouse
Concentration:
~0.05mg/ml
Immunogen:
Recombinant AAV-2 Rep 78 protein.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to 1ml final volume.
Specificity:
Antigen Recognized AAV-2, found in human in Species (tested so far). Reacts with Rep proteins (Rep78, Rep68, Rep52 and Rep40) of human AAV-2-infected cells. A weak crossreactivity is seen in some human cell lines.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Wistuba A.,Weger S., Kern A., and Kleinschmidt J. (1995) Intermediates of adeno-associated virus type 2 assembly: Identification of soluble complexes containing Rep and Cap proteins. J. Virol. 69, 5311-5319.