Description:
Applications:
Suitable for use in Immunoblot.
Recommended Dilution:
Immunoblot: 1:50-1:100
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 5F106 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 50ug | | -20°C | Blue Ice | | Mouse |
|
| Concentration: |
| ~0.05mg/ml |
| Immunogen: |
| Recombinant AAV-2 Rep 78 protein. |
| Purity: |
| Purified by Protein A affinity chromatography. |
| Form |
| Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to 1ml final volume. |
| Specificity: |
| Antigen Recognized AAV-2, found in human in Species (tested so far). Reacts with Rep proteins (Rep78, Rep68, Rep52 and Rep40) of human AAV-2-infected cells. A weak crossreactivity is seen in some human cell lines. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
Wistuba A.,Weger S., Kern A., and Kleinschmidt J. (1995) Intermediates of adeno-associated virus type 2 assembly: Identification of soluble complexes containing Rep and Cap proteins. J. Virol. 69, 5311-5319.
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