Technical Data
A0868-37C
Adeno-Associated Virus 2 Replicase (AAV2 Replicase)
Description:
Applications:
Suitable for use in Immunoprecipitation and Immunofluorescence microscopy.

Recommended Dilution:
Immunofluorescence microscopy: 1:10

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15F137Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Recombinant AAV-2 Rep78 protein, N-terminally truncated by 171aa.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to 1ml final volume.
Specificity:
Antigen recognized AAV-2, found in human in species (tested so far) in different concentrations during infection. Reacts with Rep proteins (Rep78 and Rep52) of human AAV-2-infected cells, does not react with Rep68 and Rep40. Four Rep proteins (Rep78, Rep68, Rep52 and Rep40) are expressed.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Wistuba A.,Weger S., Kern A., and Kleinschmidt J. (1995) Intermediates of adeno-associated virus type 2 assembly: Identification of soluble complexes containing Rep and Cap proteins. J. Virol. 69, 5311-5319.
Wistuba A., Kern A., Weger S., Grimm D., and Kleinschmidt J. (1997) Subcellular compartmentalization of adeno-associated virus type 2 assembly. J. Virol. 71, 1341-1352.