Suitable for use in Immunoprecipitation and Immunofluorescence microscopy.
Immunofluorescence microscopy: 1:10
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Recombinant AAV-2 Rep78 protein, N-terminally truncated by 171aa.|
|Purified by Protein A affinity chromatography.|
|Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to 1ml final volume.|
|Antigen recognized AAV-2, found in human in species (tested so far) in different concentrations during infection. Reacts with Rep proteins (Rep78 and Rep52) of human AAV-2-infected cells, does not react with Rep68 and Rep40. Four Rep proteins (Rep78, Rep68, Rep52 and Rep40) are expressed.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Wistuba A.,Weger S., Kern A., and Kleinschmidt J. (1995) Intermediates of adeno-associated virus type 2 assembly: Identification of soluble complexes containing Rep and Cap proteins. J. Virol. 69, 5311-5319.|
Wistuba A., Kern A., Weger S., Grimm D., and Kleinschmidt J. (1997) Subcellular compartmentalization of adeno-associated virus type 2 assembly. J. Virol. 71, 1341-1352.