Suitable for use in Western Blot Other applications not tested.
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| ~0.05mg/ml (after reconstitution)|
|Recombinant protein corresponding to the AAV-2 Rep78 protein, N-terminally truncated by 171aa.|
|Purified by Protein A affinity chromatography.|
|Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml of sterile ddH2O.|
|Recognizes replicase proteins (Rep78, Rep68, Rep52 and Rep40) of human AAV-2 infected cells. |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Wistuba, A., et al., (1995), “Intermediates of adeno-associated virus type 2 assembly: Identification of soluble complexes containing Rep and Cap proteins”, J. Virol., 69, 5311-5319. 2. Wistuba, A., et al., (1997), “Subcellular compartmentalization of adeno-associated virus type 2 assembly”, J. Virol., 71, 1341-1352.|