		Technical Data
A0869-58	Adenosine A2a Receptor

Description:
The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis. Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs). Based upon, biochemical and pharmacological criteria, ARs have been classified into A1, A2a, A2b , and A3. The high affinity receptor A1 inhibits adenyl cyclase, whereas low affinity receptor A2a stimulates the cyclase via G proteins. A2a receptor is believed to cause vasorelaxation in coronary artery. Various ARs and their subtypes have recently been cloned from several species. ARs belong to the superfamily of G-protein coupled receptors and predicted to contain 7 transmembrane domains. The N-termini are predicted to be extracellular and the C-termini cytoplasmic. There is an overall 30% homology among the four ARs. ARs are distributed throughout the body.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:50,000
Immunohistochemistry (Frozen): 10-50ug/ml
Western Blot: 1-10ug/ml (Chemiluminescence technique), 40-100ug membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Canine	Rabbit

Concentration:

~1mg/ml

Immunogen:

Amino acid sequence near the 4th C-terminal intracellular domain (30 amino acids) of the cloned canine A2a receptor. The A2a sequence selected is not present in A1, A2b and A3 receptors.

Purity:

Purified by affinity chromatography.

Form

Supplied as a liquid in PBS ,pH 7.4, 0.1% BSA, 0.05% sodium azide.

Specificity:

Recognizes canine Adenosine A2a receptor. Recognizes a band of ~45kD in human (coronary artery), and rat, porcine and bovine striatal membranes. Species Crossreactivty: human, rat, bovine, porcine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Science 244: 569-572 (1989). 2. Ann. Rev. Pharmacol. 35: 581-606 (1995). 3. FASEB J. 8: A850 (1994). 4. Cardiovascular Res. 27: 62-67 (1993). 5. Marala, R.B. and Mustafa, S.J., J. Pharmacol. Exp. Ther. 286: 1051-1057 (1998).