Technical Data
A0876-50A
Adenovirus
Description:
Adenoviruses are 65-80 nm, non-enveloped, regular icosahedron pathogens often associated with respiratory and gastrointestinal illness as well as conjunctivitis. Well over 40 types of adenoviruses have currently been recognized. In addition to their deleterious effects, adenoviruses have been used in vaccine production and in gene therapy. They also have the ability to trigger cell proliferation by interfering with host cellular anti-oncogenes. Since they are easy to prepare, multiple quickly and efficiently, and have the ability to infect and deliver their genome (including researcher-created constructs) to the nucleus of a variety of post-mitotic cells, adenoviruses are extremely valuable in research and medical applications ranging from virus/host cell interaction to the insertion of novel genes or regulatory mechanisms into eukaryotic cellular systems, to delivery of therapeutic anti-cancer genes.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunofluorescence: Acetone-fixed cell preparations or tissues.
Flow Cytometry: Dilute with buffer, pH 7.5-8.0 to desired working volume. For extensive dilution, protein containing or other stabilizing medium should be used.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k2Q1845Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Adenovirus 3 extract
Purity:
Purifed by Protein A affinity chromatography
Form
Supplied as a liquid in 20mM PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes the hexon protein common to all 41 serotypes of Adenovirus. Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhang Y, Huang W, Ornelles DA, Gooding LR (2010) J Virol: Modeling adenovirus latency in human lymphocyte cell lines. 2. Hierholzer, JC et al. (1987) Clin. Microbiol., 25(9):1662-1667 (1987) 3. Hierholzer, JC et al. (1984) Arch. Virol., 80:1-10 (1984) 4. McNees, AL et al. (2004) J Virol., 78(13):6955-6966 (2004).