Technical Data
A0876-50H
Adenovirus (Biotin)
Description:
The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The adenovirus early gene product E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth-inhibitory effects of TGF-beta. The E1A region encodes a series of related proteins (35-46kD) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.

Applications:
Suitable for use in Immunohistochemistry, ELISA, Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:8000
ELISA: 1:5000
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml4°C (-20°C Glycerol)Blue IceGoat
Concentration:
~4-5mg/ml
Immunogen:
Purified, disrupted Adenovirus Type 5 virions.
Purity:
Purified IgG fraction to a purity of > 95%.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins have been added. Labeled with Biotin.
Specificity:
Recognizes numerous Adenovirus proteins, including hexon. Does not crossreact with Para 1-3, Infl. A & B ,RSV, hMPV or Rhinovirus (type 16). Uninfected Cell Reactivity: Negative vs. HE-p2, LLCMK2 and Vero cells by indirect immunofluorescence. Reactive with types 2, 3, 4, 5 & 6. Broad reactivity with most/all Adeno types expected.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.