Technical Data
A0879
Adenovirus, Type 2 E1A
Description:
The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The adenovirus early gene product E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth-inhibitory effects of TGF-beta. The E1A region encodes a series of related proteins (35-46kD) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.

Applications:
Suitable for use in Immunofluorescence, Immunohistochemistry (A0878 is better), Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 2-4ug/ml for 30 minutes at RT. Staining formalin-fixed tissue requires boiling tissue section in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Immunoprecipitation (Native verified): 2ug/mg protein lysate; Use Protein A. Immunoprecipitates E1A associated proteins.
Western Blot: 1ug/ml at RT for 2 hours.
Optimal dilutions to be determined by the researcher.

Positive Control:
293 cells or adenovirus infected tissues

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k0.N.7Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceMouse
Concentration:
~0.2mg/ml
Immunogen:
Adenovirus. MW of Antigen: 35-46kD. Cellular Localization: Nuclear
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See A0879X.
Specificity:
Recognizes Adenovirus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Harlow, E., et al., Mol. Cell Biol. 6: 1579-1589 (1986). 2. Harlow, E., et al., J. Virol. 3: 533-546 (1985). 3. Whyte, P., et al., Nature 334: 124-129 (1988). 4. Klein, G., et al., Science 238: 1539-1544 (1987).