Technical Data
A0880-19A
Adenovirus Fiber (Monomer and Trimer)
Description:
Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta-sheet and beta-bends, and a knob at the C-terminus that contains the type-specific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the virion.

Applications:
Suitable for use in Immunofluorescence and Western Blot. Not suitable for the Inhibition of Ad2 attachment. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Positive Control:
Adenovirus infected cells and tissues

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
MabIgG2a,k3F13Affinity Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceMouse
Concentration:
~0.2mg/ml
Immunogen:
UV-irradiated Ad2 virus. Epitope: N-terminus, probably the first 17aa. Cellular Localization: Nuclear.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See A0880-19AX.
Specificity:
Recognizes both monomers and trimers of Adenovirus, as judged by reactivity with both boiled (monomers (62kD) only) and unboiled (both monomers and trimers (180-200kD bands)) fiber on Western Blots and by indirect Immunofluorescence studies of cells infected with a temperature-sensitive Ad5 fiber mutant (H5ts142) which does not form trimers at the nonpermissive temperature at both 32°C and 39.5°C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hong, J.S. & Engler, J.A., Virology 185: 758–767 (1991). 2. Hong, J.S. & Engler, J.A., J. Virology 70: 7071–7078 (1996).