Technical Data
A0880-19A1
Adenovirus Fiber (Monomer and Trimer)
Description:
Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta-sheet and beta-bends, and a knob at the C-terminus that contains the type-specific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the viron.

Applications:
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Positive Control:
Adenovirus infected cells and tissues.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k8J194Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceMouse
Concentration:
~0.2mg/ml
Immunogen:
UV irradiated Ad2 virus. Epitope: N-terminus. Cellular Localization: Nuclear
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity:
Recognizes both Adenovirus Fiber monomers and trimers, as judged by reactivity with both boiled (monomers (62kD) only) and unboiled (both monomers and trimers (180-200kD bands)) fibers on Western Blots and by indirect immunofluorescence studies of cells infected with a temperature-sensitive Ad5 fiber mutant which does not form trimers at the nonpermissive temperature at both 32C and 39.5C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hong, J.S., Engler, J.A. Virology., 185, 758-767, 1991.Material Safety Data: 2. Hong, J.S., Engler, J.A. J. Virology, 70, 7071-7078, 1996.