Technical Data
Adenovirus Type 9 E4 Orf1(Ad-9 E4 Orf1)
The many different serotypes of human adenoviruses (Ad) are divided into six subgroups, of which all Ad subgroup A and B and two subgroup D Ads can elicit tumors in infected rodents. Unlike the Ads from subgroup A and B, the ones from subgroup D, Ad9 and Ad10 elicit estrogen-dependent mammary tumors as opposed to undifferentiated sarcomas. In the case of Ad9, its tumorigenicity is dependent on the product of the open reading frame (ORF) 1 of the early region 4 (E4). The tumorigenic potential of Ad9 E4 Orf1 depends on a carboxyl-terminal PDZ domain-binding motif that mediates interactions with several different membrane-associated cellular proteins such as MUPP1, PATJ, MAGI-1, ZO-2 and Dlg1. It has been suggested that Ad9 E4 Orf1 may have evolved from an ancestral cellular dUTP pyrophosphatase.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ug-20CBlue IceRabbit
As reported
Synthetic peptide corresponding to 16aa from near the carboxy terminus of the Ad-9 E4 Orf1.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes Adenovirus-9 E4 Orf1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shenk T. Adenoviridae: the viruses and their replication, p. 2265-2300 in DM Knipe and PM Howley (ed.) Fields virology, 4th ed. Vol. 2. 2001. Lippincott-Raven Publishers, Philadelphia, PA. 2. Javier R, Rasha Jr. K, Macdonald GJ, et al. Human adenovirus type 9-induced rat mammary tumors. J. Virol. 1991; 65:3192-202. 3. Javier RT. Adenovirus type 9 E4 open reading frame 1 encodes a transforming protein required for the production of mammary tumors in rats. J. Virol. 1994; 68:3917-24. 4. Weiss RS, Lee SS, Prasad BV, et al. Human adenovirus early region 4 open reading frame 1 genes encode growth-transforming proteins that may be distantly related to dUTP pyrophosphatase enzymes. 1997; J. Virol. 1997; 71:1857-70.