Technical Data
A0882-62
ADK, aa22- 362, Recombinant, Human (Adenosine 5'-phosphotransferase, Adenosine Kinase, AK)
50ug
Molecular Biology Storage: -20CShipping: Blue Ice
Adenosine kinase, also known as ADK is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents.

Recombinant protein corresponding to aa22-362 from human ADK fused to a 6-His tag at the N-terminal expressed in E. coli (NP_006712).

Amino Acid Sequences:
MGSSHHHHHH SSGLVPRGSH MRENILFGMG NPLLDISAVV DKDFLDKYSL KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS LIANLAAANC YKKEKHLDLE KNWMLVEKAR
VCYIAGFFLT VSPESVLKVA HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR TGCTFPEKPD FH

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
40.5kD
Source: E. coli
Purity: ~95% (SDS-PAGE)
Concentration: ~0.5mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 20% glycerol, 1mM EDTA, 50mM sodium chloride.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Chakraborty, A., et al., J. Biol. Chem. 277: 47,451-47,451 (2002). 2. Sakowicz, M., et al., Acta Biochim. Pol. 48(3): 745-754 (2001).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.