		Technical Data
A0882-63A	ADK, CT (Adenosine Kinase, AK, Adenosine 5'-phosphotransferase)

Description:
Adenosine kinase is an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the C-terminal region of human ADK (KLH).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human ADK.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Szkotak, A.J., et al., J. Membr. Biol. 192(3):169-179 (2003). 2.Singh, B., et al., DNA Cell Biol. 20(1):53-65 (2001). 3.Van Rompay, A.R., et al., Eur. J. Biochem. 261(2):509-517 (1999). 4.Mathews, I.I., et al., Biochemistry 37(45):15607-15620 (1998). 5.McNally, T., et al., Biochem. Biophys. Res. Commun. 231(3):645-650 (1997).