Technical Data
A0887-05
Adipophilin (ADFP, Adipose Differentiation-related Protein)
Description:
Adipophilin is present in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir. Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. New specific marker for liposarcoma.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applicatons not tested.

Recommended Dilution:
Western Blot: 1:2000
Immunohistochemistry (Frozen, Paraffin embedded tissue after microwave treatment): 1:100; Incubate for 1hr at RT or overnight at 4°C.
Optimal dilutions to be determined by the reseracher.

IHC Positive Controls:
Glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertolis cells of the testis, fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver (adipocytes are negative), lipid storing CD68-positive macrophages.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceGuinea pig
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to the N-terminal (aa1-29) of human and murine Adipophilin.
Purity:
Serum
Form
Supplied as a liquid in 0.09% sodium azide.
Specificity:
Recognizes Adipophilin at ~52kD. Reactive with cell lines: Caco, PLC, HaCat, SV80, RD 125, Huvec (human umbilical cord endothelia), RV, PC-12 (rat adrenal grand), MDCK. Does not react with glioma. Species Crossreactivity: Human, mouse, rat, canine, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Heid, H.W., et al., Cell Tissue Res. 294: 309-321 (1998). 2. Heid, H.W., et al., Biochem. J. 320: 1025-1030 (1996). 3. Mrozinzki, J., et al., Adipophilin and perilipin, two lipid droplet-associated proteins, in the pathohistological differential diagnosis of human soft tissue sarcomas. Abstract from AEK meeting 1999, German Cancer Research Center. J. Cancer Res. & Clin. Oncol. 125 suppl. (1999). 4. Steiner, S., et al., Biochem. Biophys. Res. 218: 777-782 (1996). 5. Hope, R.G. and McLauchlan, J.J., General Virology 81: 1913-1925 (2000). 6. Ohsaki, Y., et al., Histochem. Cell Biol. 124: 445-452 (2005).