Technical Data
A0888-35
Adipose Triglyceride Lipase (ATGL, Desnutrin, Phospholipase A2 zeta)
Description:
Triglycerides are the most efficient form of energy storage in mammalian adipose tissue during times of caloric excess. ATGL is one of the key enzymes involved in the mobilization of fatty acids from triglyceride stores in adipose tissue, catalyzing the conversion of triacylglycerols to diacylglycerols. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity, and thus may be a potential drug target for the diabetic pathology. ATGL mRNA is detected in a wide range of tissues including adipose, lung, skeletal muscle, testis, heart, brain, and kidney, with adipose tissue expressing the highest level.
Human ATGL is 504 amino acids in length with an estimated molecular weight of 55.2kD.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin and frozen sections): 4ug/ml.
Western Blot: 1:200. Detects a band at 56kD from tissues and cells such as brown fat, liver, murine macrophages, and HepG2 cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa382-400 KRKLGRHLPSRLPEQVELR of human ATGL. Species Sequence Homology: mouse - 89.47% (17/19aa) [KRKLGdHLPSRLsEQVELR]
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.4, 0.5mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human ATGL. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zimmermann, R., et al., Science 306: 1383-1386 (2004). 2. Villena, J.A., et al., J. Biol. Chem. 279(45): 47,066-47,075 (2004).