Technical Data
A0888-35B
Adipose Triglyceride Lipase (ATGL, Desnutrin, Phospholipase A2 zeta)
Description:
Lipolytic enzymes are required for mobilization of fatty acids from triglyceride stores in adipose tissue. Energy homeostasis is affected by dysfunctional lipolysis and may contribute to the pathogenesis of obesity and insulin resistance. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. It is now thought that a second enzyme, adipose triglyceride lipase (ATGL), catalyzes the initial step in triglyceride hydrolysis. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.

Cellular Localization: Adipocytes

Positive Control: Human adipose tissue lysates and mouse 3T3-L1 cell lysates

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide within the C-terminal region [residues 400-500] of the human ATGL protein. Homology: 100% with human
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2.
Specificity:
Species Crossreactivity: This antibody reacts with human protein. Although it detects mouse protein, it does so weakly. Other species have not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Haemmerle, G., et al. Science. 312: 734-737(2006)