Technical Data
A0890-02
Adipsin, Human (Control Protein for Western Blot)
50ul
Molecular Biology Storage: -20CShipping: Blue Ice
Control Peptide for A0890 (affinity purified antibody).

Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. The switch from energy storage to expenditure is finely regulated by a variety of hormones. In order to accomplish these complex tasks energy balance, adipocytes express many genes, including adipsin, involved in lipid metabolism and glucose homeostasis. Many of these genes are finely regulated during adipocyte differentiation and maturation. Several adipocyte-derived proteins act in an autocrine or paracrine fashion to control its own and other cell's cellular physiology.

Adipsin is a serine protease that is secreted by adipocytes. It is deficient in several animal model of obesity. Adipsin has now been identified as the same protein as complement factor D. Adipsin, also called ADN or complement factor D or C3 convertase activator or properdin factor D (precursors: mouse 259aa; rat 263aa, human 253aa, mature protein 26-253, ~22kD) cleaves factor B when the latter is complexed with factor C3B, activating the C3BB complex, which then becomes C3 convertase of the alternative pathway. Adipsin is the major protein secreted by the adipocytes. Unlike rodents, adipsin is also expressed in monocytes/macrphages. Most adipsin is secreted in blood (50ug/ml in normal lean mice and 50-100 fold less in fat from db/db or ob/ob or MSG (monosodium glutamate-treated mice). Its expression is induced upon differentiation of preadipocytes.

Applications:
Suitable for use in Western Blot. Not suitable for use in ELISA or other applications where native protein is required as this preparation is not biologically active. Other applications not tested.

Recommended Dilution:
Western Blot: Load 10ul/lane. Heat prior to loading.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Products:
A0890-03: Adipsin Pab Rb xRt (affinity purified antibody)
A0890-04: Adipsin, Rat (Blocking Peptide)
A0890-05: Adipsin Pab Rb xRt (antiserum)
Source: Human
Purity: Purified ( 95%).
Concentration: Not determined
Form: Supplied as a liquid in SDS-PAGE sample buffer (reduced).
Specificity: Human adipsin/Factor D from human serum (Ezymatically inactive).

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Zhu, L., et al., J. Clin. Nvest. 94: 1163 (1994). 2. Baker, B.C., et al., Biochem. J. 279: 775 (1991). 3. Min, H.Y., et al., Nucl. Acid Res. 14: 8879. 4. White, R.T., et al., JBC 267: 9210 (1992). 5. Niemann, M.A., et al., Biochem. 23: 2482 (1984). 6. Johnson, D.M., et al., Bicohem. J. 187: 863 (1980). 7. Volanakis, J.E., et al., PNAS 77: 1116 (1980).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.