Technical Data
ADPN (Adiponutrin, Patatin-like Phospholipase Domain containing 3)
Human ADPN (Adiponutrin; also PNPLA3 and patatin-like phospholipase domain-containing protein 3) is a 53kD member of the PNPLA family of phospholipase A2 enzymes. It is expressed in white adipocytes. Adiponutrin is elevated in response to insulin and appears to act not as a phospholipase, but a molecule involved in anabolic pathways. ADPN is likely to be a type II transmembrane protein that is 481aa in length. It contains one patatin domain (aa10-179) that
is associated with in vitro enzyme activity and a transmembrane segment between
aa42-62. There appear to be two splice variants. One shows a four aa deletion of aa59-62 and a second shows an alternate start site at Met379. Over aa 160-349, human ADPN shares 61% aa identity with mouse Adiponutrin.

Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 1.0ug/ml
Immunohistochemistry: 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanSheep
Recombinant human ADPN, aa160-349 (E. coli).
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Recognizes human ADPN.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.