Technical Data
A0901-25F
Adiponutrin (PNPLA3, Patatin-like Phospholipase Domain-containing Protein 3, Acylglycerol O-acyltransferase, Calcium-independent Phospholipase A2-epsilon, iPLA2-epsilon, Pnpla3, Adpn)
Description:
Multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:128,000
Immunohistochemistry (Paraffin): 4-6ug/ml
Western Blot: 0.05-0.2ug/ml, observed in mouse heart lysates on ~45kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to EHDICPKVKSTN, from mouse PNPLA3, at the internal region of the protein (NP_473429.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes mouse PNPLA3. Species sequence homology: Canine, human, porcine and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Steinberg GR, Kemp BE, Watt MJ. Adipocyte triglyceride lipase expression in human obesity. Am J Physiol Endocrinol Metab. 2007 Oct;293(4):E958-64. Epub 2007 Jul 3.