Suitable for use in Western Blot. Other applications not tested.
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
HEK293, A431 and HepG2 cell lysates
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Synthetic peptide corresponding to C-EGLDWLSNQLRNQK, from the C-terminus of the protein sequence according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1.|
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.|
|Recognizes human ARF1, 2, 3, 4. Species sequence homology: canine, mouse, rat.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Dubois T, Paleotti O, Mironov AA, Fraisier V, Stradal TE, De Matteis MA, Franco M, Chavrier P. Golgi-localized GAP for Cdc42 functions downstream of ARF1 to control Arp2/3 complex and F-actin dynamics. Nat Cell Biol. 2005 Apr;7(4):353-64. Epub 2005 Mar 27. PMID: 15793564.|