Technical Data
A0901-81
ARF1, Recombinant, Human (ADP-ribosylation Factor 1)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
ARF1, also known as ADP-ribosylation factor 1, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. It is essential and ubiquitous in eukaryotes, as this protein is involved in vesicular transport and functioning via phospholipase D activation. ARF1 plays a role in membrane traffic and organelle integrity and are intimately tied to their reversible association with membranes and distinct interactions with membrane phospholipids.

Source:
Recombinant corresponding to aa1-181 of human ARF1 protein, fused to His-tag at N-terminus, expressed in E.coli.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K

Molecular Weight: 22.8kD (201aa) confirmed by MALDI-TOF

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
22.8kD
Source: E.coli
Purity: ~90% (SDS-PAGE).
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 20% glycerol, 0.1M sodium chloride, 1mM DTT.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Lee CM.,. et al. (1992) J Biol Chem. 267(13):9028-34.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.