Technical Data
A0902-01E
ADP Ribosylation Factor 6, aa1-175, Recombinant, Human (ARF6, DKFZp564M0264)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
ADP-ribosylation factor 6, also known as ARF6, is a member of the ADP ribosylation factor family of GTP-binding proteins. It is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. Additionally, ARF6 play a central role in maintenance of organelle integrity, assembly of coat proteins and activation of phospholipase D.

Source:
Recombinant corresponding aa1-175 of human ARF6, fused to His-tag at N-terminus, expressed in E. coli (NP_001654).

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGKVLSKIFG NKEMRILMLG LDAAGKTTIL YKLKLGQSVT TIPTVGFNVE TVTYKNVKFN VWDVGGQDKI RPLWRHYYTG TQGLIFVVDC ADRDRIDEAR QELHRIINDR EMRDAIILIF ANKQDLPDAM KPHEIQEKLG LTRIRDRNWY VQPSCATSGD
GLYEGLTWLT SNYKS

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
22.2kD
Purity: ~95% (SDS-PAGE)
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 2mM DTT, 20% glycerol, 0.2mM PMSF.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Amor J C., et al. (1994) Nature. 372:704-708. 2. Randazzo P A., et al. (1994) J Biol Chem. 269:29490-29494.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.