Technical Data
A0902-10A
ADP Ribosylation Factor (ARF)
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Technical Data |
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A0902-10A |
ADP Ribosylation Factor (ARF) |
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Description: The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21kD proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. Applications: Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation. Recommended Dilution: Immunohistochemistry: 1:500 Western Blot: 1:500 Note: Not suitable for neutralizing ARF in solution. Cellular Localization: Golgi apparatus Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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