Technical Data
ADP Ribosylation Factor (ARF)
The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21kD proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.

Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation.

Recommended Dilution:
Immunohistochemistry: 1:500
Western Blot: 1:500
Note: Not suitable for neutralizing ARF in solution.

Cellular Localization: Golgi apparatus

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanMouse
Not determined
Recombinant human ARF1 (ADP-ribosylation factor-1).
Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
Recognizes human, mouse, rat, dog and hamster ARF1, ARF3, ARF5 and ARF6. Detects a band of ~21kD by Western blot.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane: M.M. Cavenagh, et al.; J. Biol. Chem. 271, 21767 (1996)