Technical Data
ADP Ribosylation Factor Interacting Protein 2 (ARFIP2, Arfaptin 2, Partner of RAC1, POR1)
Arfaptin 2, or ADP-ribosylation factor interacting protein 2, is a Rac1 binding protein necessary for Rac-mediated actin polymerization and the subsequent formation of membrane ruffles and lamellipodia.1 Arfaptin-2 has been shown to be involved in interactions between the Rac and ARF signaling pathways.2 Arfaptin 2 also regulates the aggregation of mutant Huntingtin protein by possibly impairing proteasome function. Expression of Arfaptin 2 was shown to be increased at sites of neurodegeneration. Mutant Huntingtin protein is characteristic of Huntington’s disease, an inherited neurodegenerative disorder.3

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgYAffinity Purified
50ug-20°CBlue IceHumanChicken
As reported
Synthetic peptide corresponding to aa100-341
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Recognizes human ADP Ribosylation Factor Interacting Protein 2. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Joneson T, et al. Science 274: 1374-1376, 1996. 2. Shin OH and Exton JH. Biochem Biophys Res Comm 285:1267-1273, 2001. 3. Peters PJ, et al. Nat Cell Biol 4(3):240-245, 2002.