Technical Data
A0902-51A
ADP Ribosylation Factor Interacting Protein 2 (ARFIP2, Arfaptin 2, Partner of RAC1, POR1)
Description:
Arfaptin 2, or ADP-ribosylation factor interacting protein 2, is a Rac1 binding protein necessary for Rac-mediated actin polymerization and the subsequent formation of membrane ruffles and lamellipodia.1 Arfaptin-2 has been shown to be involved in interactions between the Rac and ARF signaling pathways.2 Arfaptin 2 also regulates the aggregation of mutant Huntingtin protein by possibly impairing proteasome function. Expression of Arfaptin 2 was shown to be increased at sites of neurodegeneration. Mutant Huntingtin protein is characteristic of Huntington’s disease, an inherited neurodegenerative disorder.3

Applications:
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 0.3-7ug/ml
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control: human HeLa cervical carcinoma, A549 lung carcinoma, MCF-7 breast adenocarcinoma, T47D breast ductal carcinoma, NCI-H69 lung carcinoma, rat PC12, and GFP-Arfaptin 2-transfected HeLa cell lysates.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~0.25 mg/ml
Immunogen:
Synthetic peptide derived from the N-terminal region of the human Arfaptin 2 protein (ADP-ribosylation factor interacting protein 2, AFP2, Partner of Rac1, or POR1)
Purity:
Purified by immunoaffinity chromatography
Form
Supplied as a liquid in PBS, pH7.4, 0.1% sodium azide.
Specificity:
Recognizes human Arfaptin 2 protein at ~38kD. Also bands observed at ~23, ~35, and ~150kD in some lysate samples. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Joneson T, et al. Science 274: 1374-1376, 1996. 2. Shin OH and Exton JH. Biochem Biophys Res Comm 285:1267-1273, 2001. 3. Peters PJ, et al. Nat Cell Biol 4(3):240-245, 2002.