		Technical Data
A0902-51A	ADP Ribosylation Factor Interacting Protein 2 (ARFIP2, Arfaptin 2, Partner of RAC1, POR1)

Description:
Arfaptin 2, or ADP-ribosylation factor interacting protein 2, is a Rac1 binding protein necessary for Rac-mediated actin polymerization and the subsequent formation of membrane ruffles and lamellipodia.1 Arfaptin-2 has been shown to be involved in interactions between the Rac and ARF signaling pathways.2 Arfaptin 2 also regulates the aggregation of mutant Huntingtin protein by possibly impairing proteasome function. Expression of Arfaptin 2 was shown to be increased at sites of neurodegeneration. Mutant Huntingtin protein is characteristic of Huntington’s disease, an inherited neurodegenerative disorder.3

Applications:
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 0.3-7ug/ml
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control: human HeLa cervical carcinoma, A549 lung carcinoma, MCF-7 breast adenocarcinoma, T47D breast ductal carcinoma, NCI-H69 lung carcinoma, rat PC12, and GFP-Arfaptin 2-transfected HeLa cell lysates.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25 mg/ml

Immunogen:

Synthetic peptide derived from the N-terminal region of the human Arfaptin 2 protein (ADP-ribosylation factor interacting protein 2, AFP2, Partner of Rac1, or POR1)

Purity:

Purified by immunoaffinity chromatography

Form

Supplied as a liquid in PBS, pH7.4, 0.1% sodium azide.

Specificity:

Recognizes human Arfaptin 2 protein at ~38kD. Also bands observed at ~23, ~35, and ~150kD in some lysate samples. Species Crossreactivity: rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Joneson T, et al. Science 274: 1374-1376, 1996. 2. Shin OH and Exton JH. Biochem Biophys Res Comm 285:1267-1273, 2001. 3. Peters PJ, et al. Nat Cell Biol 4(3):240-245, 2002.