Technical Data
A0906-01
Adrenaline (Biotin)
Description:
Applications:
Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:1000-1:2500
Immunocytochemistry: 1:1000-1:2500
Optimal dilutions to be determined by the researcher.

Ab:Biotin Label Ratio:
1:4.7

Specificity:
Using a conjugate Adrenaline-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:

Compound Crossreactivity ratio (a):
Adrenaline-G-BSA 1
Noradrenaline-G-BSA 1:140
Dopamine-G-BSA 1:170

(a) : Adrenaline-G-BSA concentration/unconjugated or conjugated catecholamine concentration at half displacement.
G = Glutaraldehyde, BSA = Bovine Serum Albumin

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Adrenaline-Glutaraldehyde-Carriers (1).
Purity:
Purified by affinity chromatography (G200).
Form
Supplied as a liquid in PBS, pH 7.2, before the addition of 40% glycerol. No preservative added. Labeled with Biotin.
Specificity:
Recognizes Adrenaline. See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.