Technical Data
A0906-91C
Adrenergic Receptor, beta 3 (B3AR, BAR3, ADRB3R, Beta-3 adrenoreceptor)
Description:
ADRB3 is a transmembrane protein and member of the G-protein coupled receptor 1 family primarily expressed by adipose tissue, which acts as a regulator of lipolysis and heat production. The ADRB3 variant Arg-64 has been identified as a possible candidate gene linked with obesity, and to an increased susceptibility to non-insulin-dependent diabetes mellitus (NIDDM).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:128,000
Western Blot: 0.03-0.1ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-28V IgG, Fc (HRP) Pab RbxGt

Recommended Useful Reagents:
HUMAN BRAIN TOTAL PROTEIN LYSATE
HUMAN CEREBELLUM, LEFT TOTAL PROTEIN LYSATE
HUMAN CEREBELLUM, RIGHT TOTAL PROTEIN LYSATE

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide sequence PHENSSLAPWPD-C from the N-Terminal region of human ADRB3.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% Sodium Azide.
Specificity:
Recognizes an epitope within the NT region of human Beta-3 adrenergic receptor, otherwise known as ADRB3 in human brain cell lysates at ~ 45kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.