Technical Data
A0906-92A
Adrenergic Receptor, beta 3 (B3AR, BAR3, ADRB3R, Beta-3 adrenoreceptor)
Description:
The beta 3-adrenergic receptor (B3AR) is a member of the super-family of G protein-coupled receptor superfamily. They are characterized by seven putative transmembrane helices connected by hydrophilic loops. The mechanism by which the activated beta ARs transmit the signals across the plasma membrane involves the stimulation of Gs. This stimulation in turn activates adenyl cyclase. Activation of adenyl cyclase yields the second messenger cAMP. B3AR is primarily present in adipocytes The B3AR plays a significant role in the control of lipolysis and thermogenesis in the brown adipose tissue of rodents and humans. In humans, B3AR, a Trp to Arg replacement has recently been discovered at position 64. This change in the first coding exon has been correlated with increased weight gain, difficulty in losing weight, insulin resistance syndrome, and worsened diabetic situation. Higher percentages of this mutation are observed in Pima Indians (over 30%) and Japanese (20%). Chronic stimulation of the B3AR by highly selective B3AR agonist CL316,243, induces ectopic expression of UCP1 in adipose tissues and skeletal muscle. This expression may contribute to the potent anti-obesity effect of the beta 3-adrenergic agonist. Human B3AR is a 408aa membrane protein (rat/mouse, 400aa). The N-terminus is predicted to be extracellular; the C-terminus intracellular.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1-10ug/ml using ECL.
ELISA: 1:10,000-1:50,000. Using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
A0906-92: Adrenergic Receptor, beta 3, Mouse, Control Peptide

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 20aa at the C-terminus of mouse B3AR (KLH). Species Sequence Homology: rat, 85%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.
Specificity:
Recognizes mouse Adrenergic Receptor, beta 3 (B3AR). No significant homology with the human B3AR.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Barak, L.S., et al., Biochem. 34: 15,407 (1995). 2. Nahmias, C., et al., EMBO J. 10: 3721-3727 (1991). 3. Van Spronsen, A., et al., Eur. J. Biochem. 213: 1117 (1993). 4.El-Yazbi AF (2006) Am J Physiol Gastrointest Liver Physiol, Genome Res., 291: G1020 - G1030. Caveolin-1 knockout alters -adrenoceptors function in mouse small intestine