		Technical Data
A0906-95D	Adrenergic Receptor Kinase, beta 1 (bARK1, B1 AR, ARK1 beta, G Protein Coupled Receptor Kinase 2, GPCR K2, GRK2)

Description:
Adrenergic receptor kinase (beta-ARK1) also known as GRK2 is part of the G Serine /Threonine protein-coupled receptor kinase family (1). B-ARK1 specifically phosphorylates G protein-coupled receptors in their activated form (agonist-bound state). Phosphorylation occurs in the carboxyl terminal tail of the receptor(2). Additionally, phosphorylation of the receptor by B-ARK1 requires beta-Arrestin to induce complete homologous desentisization(3).

Applications:
Suitable for use in Flow Cytometry, Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:100
Western Blotting: 1:500
Immunohistochemistry: 1:100-250
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:80
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG	6k13	Supernatant

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Immunogen:

A synthetic peptide corresponding to residues in the C-terminus of human B-ARK1

Purity:

Supernatant

Form

Supplied as a liquid

Specificity:

Recognizes human B-ARK1

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Premont, R. T., Inglese, J., and Lefkowitz, R. J. (1995) FASEB J. 9, 175-182 2. Premont, R., Koch, W. J., Inglese, J., and Lefkowitz, R. J. (1994) Identification, purification, and characterization of GRK5, a member of the family of G protein-coupled receptor kinases. J. Biol. Chem. 269, 6832-6841 3. Lefkowitz, R.J. G protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization. J. Biol. Chem. 273: 18677–18680 (1998).