Technical Data
Adrenergic Receptor Kinase, beta 1 (bARK1, B1 AR, ARK1 beta, G-Protein Coupled Receptor Kinase 2, GPCR K2, GRK2)
Adrenergic receptor kinase (beta-ARK1) also known as GRK2 is part of the G Serine /Threonine protein-coupled receptor kinase family (1). B-ARK1 specifically phosphorylates G protein-coupled receptors in their activated form (agonist-bound state). Phosphorylation occurs in the carboxyl terminal tail of the receptor(2). Additionally, phosphorylation of the receptor by B-ARK1 requires beta-Arrestin to induce complete homologous desentisization(3).

Suitable for use in Flow Cytometry, Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:100
Western Blotting: 1:500
Immunohistochemistry: 1:100-250
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:80
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
A synthetic peptide corresponding to residues in the C-terminus of human B-ARK1
Supplied as a liquid
Recognizes human B-ARK1
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Premont, R. T., Inglese, J., and Lefkowitz, R. J. (1995) FASEB J. 9, 175-182 2. Premont, R., Koch, W. J., Inglese, J., and Lefkowitz, R. J. (1994) Identification, purification, and characterization of GRK5, a member of the family of G protein-coupled receptor kinases. J. Biol. Chem. 269, 6832-6841 3. Lefkowitz, R.J. G protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization. J. Biol. Chem. 273: 18677–18680 (1998).