Technical Data
A0906-95F
Adrenergic Receptor Kinase, beta 1 (bARK1, B1 AR, ARK1 beta, G Protein Coupled Receptor Kinase 2, GPCR K2, GRK2)
Description:
G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183-188 amino acids and a carboxyl-terminus of variable length. GRK2 is a member of the BetaARK subfamily, and was originally termed Beta-adrenergic receptor kinase 1 (BetaARK1).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b8H208Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
E. coli-derived rhGRK2. (aa 468 - 689)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile 40-50% glycerol, PBS.
Specificity:
Recognizes human/mouse/rat GRK2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.