Technical Data
ACTH, NT (Adrenocorticotropic Hormone, Adrenocorticotropin, Corticotropin)
Adrenocorticotropic hormone (ACTH), also known as corticotropin, is a polypeptide tropic hormone produced and secreted by the anterior pituitary gland. It is an important component of the hypothalamic-pituitary-adrenal axis and is often produced in response to biological stress (along with its precursor corticotropin-releasing hormone from the hypothalamus). Its principal effects are increased production and release of corticosteroids. A deficiency of ACTH is a cause of secondary adrenal insufficiency and an excess of it is a cause of Cushing's disease.

Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 0.2-0.4ug/ml. Incubate 30 minutes at RT. No special pretreatment required for staining.
Optimal dilutions to be determined by the researcher.

Positive Control:
Normal pituitary glad or pituitary adenoma

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG10.N.10Affinity Purified
500ul-20°CBlue IceHumanMouse
Synthetic peptide corresponding to aa1-24 from the N-terminal of human ACTH. Cellular Localization: Cytoplasmic
Purified by Protein G affinity chromatography.
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA and azide.
Recognizes human Adrenocorticotrophic Hormone (ACTH). Shows broad species crossreactivity.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hsu DW; Hooi SC; Hedley-Whyte ET; Strauss RM; Kaplan LM. Coexpression of galanin and adrenocorticotropic hormone in human pituitary and pituitary adenomas. American Journal of Pathology, 1991 Apr, 138(4):897-909. 2. Kasper M; Kasper M; Kern F; Heitz PU; Gudat F. Immunohistochemical studies on human pituitary gland and adenomas. Journal fur Hirnforschung, 1991, 32(6):725-34. 3. Berg KK; Scheithauer BW; Felix I; Kovacs K; Horvath E; Klee GG; Laws ER Jr. Pituitary adenomas that produce adrenocorticotropic hormone and alpha-subunit: clinicopathological, immuno-histochemical, ultrastructural, and immunoelectron microscopic studies in nine cases. Neurosurgery, 1990 Mar, 26(3):397-403. 4. Vrontakis ME; Sano T; Kovacs K; Friesen HG. Presence of galanin-like immunoreactivity in nontumorous corticotrophs and corticotroph adenomas of the human pituitary. Journal of Clinical Endocrinology and Metabolism, 1990, 70(3):747-51. 5. Nakane PK. Classification of anterior pituitary cell types with immunoenzyme histochemistry. J Histochemistry and Cytochemistry, 1970, 18:9-20. 6. Ueda G; Moy P; Furth J. Multihormonal activities of normal and neoplastic pituitary cells as indicated by immunohistochemical staining. International Journal of Cancer, 1973, 12:100-14. 7. Heitz PU. Multihormonal pituitary adenomas. Hormonal Research, 1979, 10:1-13. 8. Martinez D; Barthe D. Heterogeneous pituitary adenomas. A light microscopic, immunohistochemical and electron microscopic study. Virchows Arch Pathol Anat, 1982, 394:221-33. 9. Horvath E; Kovacs K. Histologic, immunocytologic, and fine structural finding in pituitary adenomas associated with the multiple endocrine neoplasia syndrome (MENS). Lab Invest, 1979, 40:261. 10. Robert F; Pelletier G; Hardy J. Pituitary adenomas in Cushing’s disease. A histologic, ultrastructural and immunocytochemical study. Arch Pathol Lab Med, 1978, 102:448-55. 11. Young LW; Lim GHK; Forbes GB et. al. Postadrenalectomy pituitary adenoma (Nelson’s syndrome) in childhood: Clinical and roentgenologic detection. Am J Roentgenol, 1976, 126:550-9. 12. Mukai K; Wolseth DG; Bonner RA, et. al. Pituitary carcinoma with liver metastases: An immunocytochemical study. Lab Invest, 1980, 42:42.