Technical Data
A0909
ACTH, NT (Adrenocorticotropic Hormone, Adrenocorticotropin, Corticotropin)
Description:
Adrenocorticotropic hormone (ACTH), also known as corticotropin, is a polypeptide tropic hormone produced and secreted by the anterior pituitary gland. It is an important component of the hypothalamic-pituitary-adrenal axis and is often produced in response to biological stress (along with its precursor corticotropin-releasing hormone from the hypothalamus). Its principal effects are increased production and release of corticosteroids. A deficiency of ACTH is a cause of secondary adrenal insufficiency and an excess of it is a cause of Cushing's disease.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 0.2-0.4ug/ml. Incubate 30 minutes at RT. No special pretreatment required for staining.
Optimal dilutions to be determined by the researcher.

Positive Control:
Normal pituitary glad or pituitary adenoma

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.N.10Affinity Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Synthetic peptide corresponding to aa1-24 from the N-terminal of human ACTH. Cellular Localization: Cytoplasmic
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA and azide.
Specificity:
Recognizes human Adrenocorticotrophic Hormone (ACTH). Shows broad species crossreactivity.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hsu DW; Hooi SC; Hedley-Whyte ET; Strauss RM; Kaplan LM. Coexpression of galanin and adrenocorticotropic hormone in human pituitary and pituitary adenomas. American Journal of Pathology, 1991 Apr, 138(4):897-909. 2. Kasper M; Kasper M; Kern F; Heitz PU; Gudat F. Immunohistochemical studies on human pituitary gland and adenomas. Journal fur Hirnforschung, 1991, 32(6):725-34. 3. Berg KK; Scheithauer BW; Felix I; Kovacs K; Horvath E; Klee GG; Laws ER Jr. Pituitary adenomas that produce adrenocorticotropic hormone and alpha-subunit: clinicopathological, immuno-histochemical, ultrastructural, and immunoelectron microscopic studies in nine cases. Neurosurgery, 1990 Mar, 26(3):397-403. 4. Vrontakis ME; Sano T; Kovacs K; Friesen HG. Presence of galanin-like immunoreactivity in nontumorous corticotrophs and corticotroph adenomas of the human pituitary. Journal of Clinical Endocrinology and Metabolism, 1990, 70(3):747-51. 5. Nakane PK. Classification of anterior pituitary cell types with immunoenzyme histochemistry. J Histochemistry and Cytochemistry, 1970, 18:9-20. 6. Ueda G; Moy P; Furth J. Multihormonal activities of normal and neoplastic pituitary cells as indicated by immunohistochemical staining. International Journal of Cancer, 1973, 12:100-14. 7. Heitz PU. Multihormonal pituitary adenomas. Hormonal Research, 1979, 10:1-13. 8. Martinez D; Barthe D. Heterogeneous pituitary adenomas. A light microscopic, immunohistochemical and electron microscopic study. Virchows Arch Pathol Anat, 1982, 394:221-33. 9. Horvath E; Kovacs K. Histologic, immunocytologic, and fine structural finding in pituitary adenomas associated with the multiple endocrine neoplasia syndrome (MENS). Lab Invest, 1979, 40:261. 10. Robert F; Pelletier G; Hardy J. Pituitary adenomas in Cushing’s disease. A histologic, ultrastructural and immunocytochemical study. Arch Pathol Lab Med, 1978, 102:448-55. 11. Young LW; Lim GHK; Forbes GB et. al. Postadrenalectomy pituitary adenoma (Nelson’s syndrome) in childhood: Clinical and roentgenologic detection. Am J Roentgenol, 1976, 126:550-9. 12. Mukai K; Wolseth DG; Bonner RA, et. al. Pituitary carcinoma with liver metastases: An immunocytochemical study. Lab Invest, 1980, 42:42.