Technical Data
A0909-60E
ADSL, Recombinant, Human, aa1-484, His-Tag (Adenylosuccinate Lyase, Adenylosuccinase, ASase, ASL, AMPS)
100ug
500ug
Molecular Biology Storage: -20CShipping: Blue Ice
ADSL, is an enzyme that converts adenylosuccinate to AMP and fumarate as part of the purine nucleotide cycle. Defects in ADSL are the cause of adenylosuccinase deficiency (ADSL deficiency). ADSL deficiency is an autosomal recessive disorder characterized by the accumulation in the body fluids of succinylaminoimidazole-carboxamide riboside (SAICA-riboside) and succinyladenosine (S-Ado).

Source:
Recombinant corresponding to aa1-484 from human ADSL, fused to His-tag at N-terminal expressed in E.coli.

AA Sequence:
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAAG GDHGSPDSYR SPLASRYASP EMCFVFSDRY KFRTWRQLWL WLAEAEQTLG LPITDEQIQE MKSNLENIDF KMAAEEEKRL RHDVMAHVHT FGHCCPKAAG IIHLGATSCY VGDNTDLIIL RNALDLLLPK LARVISRLAD FAKERASLPT LGFTHFQPAQ LTTVGKRCCL WIQDLCMDLQ NLKRVRDDLR FRGVKGTTGT QASFLQLFEG DDHKVEQLDK MVTEKAGFKR AFIITGQTYT RKVDIEVLSV LASLGASVHK ICTDIRLLAN LKEMEEPFEK QQIGSSAMPY KRNPMRSERC CSLARHLMTL VMDPLQTASV QWFERTLDDS ANRRICLAEA FLTADTILNT LQNISEGLVV YPKVIERRIR QELPFMATEN IIMAMVKAGG SRQDCHEKIR VLSQQAASVV KQEGGDNDLI ERIQVDAYFS PIHSQLDHLL DPSSFTGRAS QQVQRFLEEE VYPLLKPYES VMKVKAELCL

Molecular Weight:
~59kD

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~59kD
Source: E.coli
Purity: ~95% (SDS-PAGE)
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 1mM DTT, 40% glycerol, 0.1M sodium chloride.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Marie S., et al. (2002) Am J Hum Genet. 71:14-21. 2. Kmoch S., et al. (2000) 3. Hum Mal Genet. 9:1501-1513.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.