Technical Data
AES, CT (Amino-terminal Enhancer of Split, GRG, ESP1, TLE5)
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml; 293 cell lysate can be used as a positive control.
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

PabIgGAffinity Purified
100ug-20°CBlue IceHumanRabbit
As reported
Synthetic peptide corresponding to 16 amino acids near the carboxy terminus of human AES (Genbank accession No. NP_945320)
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.02% sodium azide.
Reacts with human, mouse and rat AES.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Martin, S.S., et al., (2004), “Regulation of Bcl-2 proteins during anoikis and amorphosis”, Biochim Biophys Acta., 1692: 145-157 2. Miyasaka, H., et al., (1993), “Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein”, Eur. J. Biochem., 216:343-352 3. Chen, G., et al., (2000), “Groucho/TLE family proteins and transcriptional repression”, Gene, 249: 1-16 4. Jan, Y., et al., (2004), “A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and groucho/TLE corepressors”, Cell, 116: 751-762