		Technical Data
A0921-81	AES, NT (Amino-terminal Enhancer of Split, GRG, ESP1, TLE5)

Description:
Description:
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop- tosis induced by integrin detachment (1). Amino-terminal enhancer of split (AES) is a member of the Groucho/
transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co- repressors that play important roles in neurogenesis, segmentation, and sex determination (2,3). AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a
mitochondrial protein that is released into the cytoplasm upon onset of apoptosis (4). It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. (4). Interestingly, apoptosis of cells transfected with AES
and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the 5 1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the 5 1 integrin (4).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug’ml
Optimal dilutions to be determined by the researcher.

Positive Control:
293 cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide AES antibody was raised against a 16 amino acid peptide from near the amino-terminus of human AES (Genbank accession No. NP_945320).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

Specificity:

Recognizes human AES. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57. 2. Miyasaka H, Choudhury BK, Hou WE, et al. Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein. Eur. J. Biochem. 1993; 216:343-52. 3. Chen G and Courey AJ. Groucho/TLE family proteins and transcriptional repression. Gene 2000; 249:1-16. 4. Jan Y, Matter M, Pai J-t, et al. A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and groucho/TLE corepressors. Cell 2004; 116:751-762. (WD1005)