		Technical Data
A0922-01	Afamin (ALB2, ALBA, ALF, Alpha-Alb, Alpalpha-albumin, MGC125339, MGC125338, ALF, ALBA, ALB2, afamin, AFMha-albumin, MGC125338, MGC125339)

Description:
This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:16,000
Western Blot: 0.3-1ug/ml detects ~80kD band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 69.1kD according to NP_001124.1)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-NKDDRPKDLSLRE, from the internal region of human Afamin (NP_001124.1).

Purity:

Purified by peptide affinity chromatography.

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Specificity:

Recognizes human Afamin. Species sequence homology: mouse, rat, canine, bovine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kratzer I, Bernhart E, Wintersperger A, Hammer A, Waltl S, Malle E, Sperk G, Wietzorrek G, Dieplinger H, Sattler W.Afamin is synthesized by cerebrovascular endothelial cells and mediates alpha-tocopherol transport across an in vitro model of the blood-brain barrier.Journal of neurochemistry 2009 Feb 108 (3): 707-18.