Technical Data
A0924-01D
Aflatoxin B1
Description:
Aflatoxin is a naturally occurring mycotoxin produced by two types of mold: Aspergillus flavus and Aspergillus parasiticus. Aspergillus flavus is common and widespread in nature and is most often found when certain grains are grown under stressful conditions such as drought. At least 13 different types of aflatoxin are produced in nature with aflatoxin B1 considered as the most toxic. Aflatoxin B1 exposure may be a potential etiological agent in hepatocellular carcinoma.

Applications:
Suitable for use in Immunofluorescence, ELISA and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Cellular Localization: Nuclear

Positive Control: human HepG2 liver samples

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k8J206Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
~1.5mg/ml
Immunogen:
Aflatoxin B1 DNA (against the imidazole ring-opened persistent form of the major N-7 guanine adduct of AFB1)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid containing sodium azide
Specificity:
Recognizes Aflatoxin B1. Species Crossreactivity: All species. There is no crossreactivity with other carcinogen-DNA adducts.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chen, CJ, et al. Aflatoxin B1 DNA adducts in smeared tumor tissue from patients with hepatocellular carcinoma. Hepatology. 16(5): 1150-1155 (1992) [uses this clone] 2. Zhang, YJ, et al. Aflatoxin B1 DNA adducts and hepatitis B virus antigens in hepatocellular carcinoma and non-tumorous liver tissue. Carcinogenesis. 12(12): 2247-2252 (1991) [uses this clone]