Technical Data
A0925-01B
Aflatoxin M1, B1 (AFM1, AFB1)
Description:
Aflatoxin is a potent carcinogen. The fungus, genus Aspergillus, that secretes aflatoxin enters the plant usually through the wounds left by an insect pest, since most plants have a hard outer layer that acts as a barrier to fungi. Once inside the plant, the fungus can reach all parts of the plant, including its seeds. Aflatoxin is a serious cause of illness and death in developing countries which have fewer food safety regulations. Some plant scientists are trying to develop transgenic corn with a gene for an enzyme which breaks down aflatoxin. Aflatoxin is listed as a possible carcinogen, and has been shown to cause liver damage, some of which may be cumulative,in many mammals that ingest sufficient quantities of it, though tolerance seems to vary by species. In most species, aflatoxin cannot be detected in the liver 14 days after it has been withdrawn from the animals diet, though some of its effects may still be present. Animals affected by aflatoxicosis show reduced feed intake, and even mortality, if sufficient amounts of aflatoxin are ingested for a sufficient period of time. Sensitivity, however, seems to vary not only by species, but also by individual animals.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b3H58Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceRat
Concentration:
~1mg/ml
Immunogen:
Winstar rats were immunized with Aflatoxin M1, conjugated to BSA.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 500ul sterile ddH2O.
Specificity:
Recognizes Aflatoxin M1 (AFM1) and Aflatoxin B1 (AFB1). Does not react to BSA and other irrelevant antigens by ELISA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.