Technical Data
A0925-01C
Aflatoxin M1, B1 (AFM1, AFB1)
Description:
Aflatoxin is a potent carcinogen. The fungus, genus Aspergillus, that secretes aflatoxin enters the plant usually through the wounds left by an insect pest, since most plants have a hard outer layer that acts as a barrier to fungi. Once inside the plant, the funguscan reach all parts of the plant, including its seeds. Aflatoxin is a serious cause of illness and death in developing countries which have fewer food safety regulations. Some plant scientists are trying to develop transgenic corn with a gene for an enzyme whichbreaks down aflatoxin. Aflatoxin is listed as a possible carcinogen, and has been shown to cause liver damage, some of which may be cumulative, in many mammals that ingest sufficient quantities of it, though tolerance seems to vary by species. In mostspecies, aflatoxin cannot be detected in the liver 14 days after it has been withdrawn from the animals diet, though some of its effects may still be present. Animals affected by aflatoxicosis show reduced feed intake, and even mortality, if sufficient amounts ofaflatoxin are ingested for a sufficient period of time. Sensitivity, however, seems to vary not only by species, but also by individual animals.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b,k3H59Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceRat
Concentration:
~1mg/ml
Immunogen:
Aflatoxin M1-BSA conjugate
Purity:
Purified by Protein G affinity chromatography.
Form
Lyophilized from a solution in 0.01MPBS, pH7.2
Specificity:
Reactive to Aflatoxin M1 (AFM1) and Aflatoxin B1 (AFB1), but not reactive to BSA and other irrelevant antigens by ELISA. Similarly, a competitive binding assay using AFM1 as binding competitor to compete with AFM1-BSA-125I showed that AFM1 can effectively inhibit the binding of the above 4 monoclonals to AFM1-BSA-125I and the inhibition degree corresponded to the amounts of AFM1 used.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.