		Technical Data
A0928	AFX, phosphorylated (Ser193)

Description:
The Forkhead family of transcription factors is involved in tumorigenesis in rhabdomyosarcoma and acute leukemias. Within the family, three members (FKHR, AFX and FKHRL1) have sequence similarity to the nematode homologue DAF- 16, which has been shown to mediate signaling via a pathway involving IGFR1, PI3K and Akt. There are three Akt phosphorylation sites in the FKHR proteins: Thr24, Ser256 and Ser319. Phosphorylation of FKHR family members at these sites by Akt promotes cell survival and regulates the cell cycle. Phosphorylation regulates FKHR nuclear translocation and target gene transcription.

Applications:
Suitable for use in Western Blot and ELISA. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Molecular Weight: 65kD

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		4°C (-20°C Glycerol)	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser193 of human AFX.

Purity:

Purified by Protein A and peptide affinity chromatography.

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA 50% glycerol. No preservative added.

Specificity:

Phospho-AFX (Ser193) Antibody detects endogenous levels of AFX only when phosphorylated at Ser193. Species Crossreactivity: Human, mouse, hampster.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

(1) Anderson, M.J. et al. (1998) Genomics 47, 187–199. (2) Galili, N. et al. (1993) Nat. Genet. 5, 230–235. (3) Borkhardt, A. et al. (1997) Oncogene 14, 195–202. (4) Nakae, J. et al. (1999) J. Biol. Chem. 274, 15982–15985. (5) Rena, G. et al. (1999) J. Biol. Chem. 274, 17179–17183. (6) Guo, S. et al. (1999) J. Biol. Chem. 274, 17184–17192. (7) Brunet, A. et al. (1999) Cell 96, 857–868. (8) Medema, R.H. (2000) Nature 404, 782–787.