Technical Data
Several studies indicate that FKHR and AFX, mammalian homologues of the Caenorhabditis elegans forkhead transcription factor DAF-16, function in the insulin signaling pathway promoter contains one IRS, while in the IGFBP-1 promoter two copies of IRS are arranged as an inverted palindrome. Three tandem copies of IRSs are present in the G6Pase. AFX has been recently shown to serve as a substrate of PKB both in vitro and in vivo, and its transcriptional activity is inhibited by phosphorylation.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthesized non-phosphopeptide derived from human AFX around the phosphorylation site of serine 197
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.
Detects endogenous levels of total AFX protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.