Technical Data
ATGL Blocking Peptide
Molecular Biology Storage: -20CShipping: Blue Ice
Peptide is used to block ATGL Rabbit mAb reactivity in immunohistochemistry protocols.

Triglycerides form an important energy store in many living organisms. Adipose tissue serves as the primary storage depot for triglycerides in mammals. Lipolytic enzymes mobilize triglycerides during periods of starvation to provide organisms with necessary energy. Hormone-sensitive lipase (HSL), the first identified lipolytic enzyme, hydrolyzes triglycerides in mammalian adipose tissues (1-3). Additional lipolytic enzymes, including adipose triglyceride lipase (ATGL), have also been discovered. The primary function of ATGL is to catalyze the hydrolysis of the first ester bond of lipid molecules. This enzyme may provide diglyceride substrates for HSL hydrolysis. ATGL is abundantly expressed in murine white and brown adipose tissue, and is highly substrate specific (4). ATGL was independently identified as desnutrin (5) and the TG-hydrolace inducible phospholipase-A2- (6).

Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks ATGL Rabbit mAb by immunohistochemistry.

Directions for Use: For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100ul total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity: Highly Purified
Form: Supplied as a liquid in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
(1) Holm, C. et al. (1988) Science 241, 15031506. (2) Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533537. (3) Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215221. (4) Zimmermann, R. et al. (2004) Science 306, 13831386. (5) Villena, J.A. et al. (2004) J. Biol. Chem. 279, 4706647075. (6) Jenkins, C.M. et al. (2004) J. Biol. Chem. 279, 4896848975.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.