Agarose DNA Fragments 50-1000bp
|BioSeparation?||Storage: RTShipping: RT|
Agarose for DNA Fragments 50-1000bp is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels.
Manufactured specifically for primer size fragments in the range of 50-1000 base pairs; exhibits exceptionally high transparency even at high concentrations (4.5%).
• Analytical DNA Gels 1000bp
• PCR products
• Restriction Enzyme digestion products
• Mutation Analysis
• Blotting Assays 600bp
• For use with all buffer systems
Recommended Usage (1X TAE):
Concentration Molecular Weight
1.8% 500-1000 bp
3.0% 150-600 bp
4.5% 50-350 bp
Note: A 3% agarose gel is equivalent to 8% polyacrylamide
Storage and Stability: Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.
Appearance: White, fine, homogenous, free flowing powder
Clarity (1.5%, NTU): ~4
Absorbance (430nm, 1.5%): 0.040
pH (1.5%, H2O, Solution): 6-7
pH (1.5%, H2O, Gel): 5.0-7.0
Loss on Drying: 7.0%
Melting Point (3%): 75°C
Gel Point (3%): 35°C
Gel Strength (1.5%): 750g/cm2
Gel Strength (3%): 1500g/cm2
RNase: None Detected
DNase: None Detected
Quality Control: Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.
Functional and Molecular Biology Testing: To Pass Test
• Comparative assay of different size DNA fragments
• Southern Blot (125-23,000bp)
• Background Fluorescence (TAE/TE, EtBr):
• DNA Binding (TAE, EtBr)
• Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase)
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Ausubel, F.M., et al., Current Protocols in Molecular Biology, John Wiley (1992). 2. Maniatis, T., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press (1989).