Agarose DNA Fragments 150-1500bp
|BioSeparation?||Storage: RTShipping: RT|
Agarose for DNA Fragments 150-1500bp is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels.
Manufactured specifically for primer size fragments in the range of 150-1500 base pairs and exhibits exceptionally high transparency even at high concentrations (4.5%).
Analytical DNA Gels 1500bp
Restriction Enzyme digestion products
Blotting Assays 600bp
For use with all buffer systems
Recommended Usage (1X TAE):
Concentration Molecular Weight
4% 150-600 bp
12% 500-1500 bp
Storage and Stability: Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.
Appearance: White, fine, free flowing powder
Clarity (NTU, 1.5%): ~1.5
Colorimetry (A450): 0.02
pH in solution (1.5%): 5.5-7.0
pH in gel (1.5%): 5.5-7.0
Gel Strength (1.5%): 2000g/cm2
Gel Strength (4%): 4250g/cm2
Gel Point (4%): 40°C
Melting Point (4%): 89ēC
Loss on Drying: 7.0%
RNase: None Detected
DNase: None Detected
Quality Control: Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.
Functional and Molecular Biology Testing: To Pass Test
Comparative assay of different size DNA fragments
Southern Blot (125-23,000bp)
Background Fluorescence (TAE/TE, EtBr):
DNA Binding (TAE, EtBr)
Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase)
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Ausubel, F. M., et al., Current Protocols in Molecular Biology, John Wiley (1992). 2. Maniatis, T., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press (1989).